Acute Changes in Arterial Stiffness with Palatinose Versus Glucose Intake in Elderly

Article Information

Ryota Kobayashi1, Kaori Sato2, Miki Sakazaki3, Yukie Nagai3, Toshihiko Takahashi4, Kenji Asaki5, Soichiro Iwanuma6, Nobuyuki Ohashi7, Takeo Hashiguchi6

1Center for Fundamental Education, Teikyo University of Science: 2-2-1 Senju, Sakuragi, Adachi-ku, Tokyo 120-0045, Japan

2College of Liberal Arts, International Christian University: 3-10-2, Osawa, Mitaka Shi, Tokyo To, 181-0015, Japan

3Research & Development Division, Mitsui Sugar Co., Ltd: 36-2 Nihonbashihakozakicho, Chuo City, Tokyo 103-8423, Japan

4Adachi-ku Track and Field Association: 1-33-22 Yanaka, Adachi-ku, Tokyo 120-0006, Japan

5Department of Tokyo Judo Therapy, Teikyo University of Science: 2-2-1 Senju, Sakuragi, Adachi-ku, Tokyo 120-0045, Japan

6Department of School Education, Teikyo University of Science: 2-2-1 Senju, Sakuragi, Adachi-ku, Tokyo 120-0045, Japan

7Department of Faculty of Medical Technology, Teikyo University: 359 Otsuka, Hachioji, Tokyo 192-0395, Japan

*Corresponding author: Ryota Kobayashi, Center for Fundamental Education, Teikyo University of Science: 2-2-1 Senju, Sakuragi, Adachi-ku, Tokyo 120-0045, Japan

Received: 08 March 2020; Accepted: 16 March 2020; Published: 20 March 2020

Citation: Ryota Kobayashi, Kaori Sato, Miki Sakazaki, Yukie Nagai, Toshihiko Takahashi, Kenji Asaki, Soichiro Iwanuma, Nobuyuki Ohashi, Takeo Hashiguchi. Acute Changes in Arterial Stiffness with Palatinose Versus Glucose Intake in Elderly. Cardiology and Cardiovascular Medicine 4 (2020): 144-153.

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Abstract

Background: We assessed acute effects of palatinose (isomaltulose) consumption on arterial stiffness. Increased arterial stiffness due to elevated postprandial blood glucose levels increases the risk of cardiovascular disease. Palatinose appears to suppress an increase in BG levels while securing energy, and might decrease acute increases in arterial stiffness during hyperglycemia. However, the acute effects of palatinose consumption on arterial stiffness are unknown.

Methods: Ten older adults had two interventions in the following cross-over design: (i) ingestion of 25 g of glucose solution (GSI trial) and (ii) ingestion of 25 g of palatinose solution (PSI trial). Participants fasted for 12 h and then visited the laboratory. We assessed brachialankle (ba) and heartbrachial (hb) pulse wave velocity (PWV), the cardio-ankle vascular index (CAVI), brachial and ankle blood pressure, heart rate, and blood glucose levels at baseline (before ingestion) and 30, 60, and 90 min after ingestion.

Results: Changes in baPWV, hbPWV, the CAVI, and ankle systolic blood pressure were significantly increased from baseline at 30, 60, and 90 min after ingestion in the GSI trial (p < 0.05), but not in the PSI trial. The changes in baPWV, hbPWV, and CAVI were significantly lower after ingestion in the PSI trial compared with the GSI trial. Blood glucose levels were significantly lower at 30 and 60 min after ingestion in the PSI trial compared with the GSI trial (p < 0.01).

Conclusions: We conclude that arterial stiffness may increase after glucose ingestion, but it is not elevated after palatinose ingestion.

Keywords

Palatinose ingestion; Arterial stiffness; Blood pressure; Blood glucose

Palatinose ingestion articles, Arterial stiffness articles, Blood pressure articles, Blood glucose articles

Article Details

Introduction

Increased arterial stiffness is an independent risk factor for development of cardiovascular disease [1, 2]. Arterial stiffness increases with age [3]. Therefore, suppressing arterial stiffness in older people is important for preventing cardiovascular disease.

Over the years, carbohydrates have obtained a bad reputation. Elevated postprandial blood glucose (BG) levels increases arterial stiffness [4]. Arterial stiffness increases after glucose ingestion in older people [5]. BG levels increase after meal with age [6]. Increased arterial wall stiffness due to a postprandial increase in BG levels is an independent risk factor for cardiovascular disease [7] [8]. Thus, acute increases in postprandial arterial stiffness in older people should be inhibited. However, moderate carbohydrate intake has been reported as necessary for health (mortality risk) [9]. Moreover, sugar is not only a basic nutrient that supports life, but also has an important role in contributing to taste, such as sweetness and texture, and to impart satisfaction to food [10]. Therefore, it is necessary to clarify a method for suppressing an increase in arterial stiffness while taking carbohydrates in the elderly.

Isomaltulose (palatinose: a registered trademark of Mitsui Sugar Co., Ltd.) is a carbohydrate that takes into account the quality of nutrition. Palatinose is a natural carbohydrate found in honey [11]. Palatinose has similar sweetness, taste, and energy as other sugars. However, the rate of degradation of palatinose in the small intestine is slow, and it can be absorbed slowly to suppress a rise in BG levels [12]. A study of 10 healthy individuals showed a slow increase in BG levels after palatinose intake, showing that peak values were lower than other sugars [13]. Therefore, palatinose appears to suppress an increase in BG levels while securing energy, and might decrease acute increases in arterial stiffness during hyperglycemia. However, the acute effects of palatinose ingestion on arterial stiffness are unknown.

The purpose of this study was to examine changes in arterial stiffness after palatinose intake in the elderly. The present study hypothesized that ingesting glucose, which causes a rapid rise in blood glucose, would increase arterial stiffness, but that arterial stiffness would not increase after ingestion of palatinose.

Materials and methods

Participants

We enrolled 10 older adults (8 women; age, 74.3 ± 3.4 years; height, 154.0 ± 3.4 cm; weight, 55.9 ± 4.3 kg) in this study. Study participants had blood pressure (BP) (<135/85 mm Hg) and fasting BG (<109 mg/dl). The participants had no history, taking, or exercise habits. All of the women who were enrolled were post-menopausal, which is important because estrogen improves arterial stiffness [14]. None of the women had received estrogen replacement therapy. Therefore, post-menopausal women were chosen to avoid the cyclical effects of hormones on arterial stiffness (Table 1). The experimental procedures and study purpose were explained to all volunteers who then provided written informed consent to participate. The study was approved by the Ethics Committee at Teikyo University of Science (18096). The study was performed in accordance with the guidelines for human experimentation published by our Institutional Review Board and the principles of the Declaration of Helsinki.

Value

Age (years)

74.3 ± 3.4

Height (cm)

154.0 ± 3.4

Weight (kg)

55.9 ± 4.3

Body fat (%)

30.4 ± 2.0

BMI (kg/m2)

23.4 ± 1.1

Brachial SBP (mmHg)

127.1 ± 5.5

Ankle SBP (mmHg)

148.1 ± 7.7

Heart rate (bpm)

63.4 ± 2.1

Fasting blood glucose (mg/dl)

90.3 ± 3.7

Muscle mass of the right upper arm (kg)

1.8 ± 0.2

Muscle mass of left upper arm (kg)

1.6 ± 0.2

Muscle mass of right leg (kg)

7.0 ± 0.6

Muscle mass of left leg (kg)

6.9 ± 0.6

Table 1: Subject characteristics

Values are mean ± SD.

BMI, body mass index; SBP, systolic blood pressure.

Study design

Each subject participated in two trials in a cross-over fashion as follows: (i) ingestion of 25 g of glucose solution (GSI trial) and (ii) ingestion of 25 g of palatinose solution (PSI trial). Each subject waited approximately 3 days after completion of one trial before crossing over to the next trial. After fasting for 12 h, subjects visited the laboratory. After resting in the supine position for 10 min, we measured arterial stiffness, brachial and ankle BP, heart rate (HR), and BG levels at baseline (before ingestion) and 30, 60, and 90 min after ingestion (Figure 1). The subjects refrained from consuming alcohol and caffeine and performing exercise for 24 h before testing. They were also asked to keep a log of all consumed foods and to repeat this diet during the second intervention. All measurements were performed at a constant room temperature of 25°C in a quiet room. Changes in lifestyle habits (eating habits, exercise, etc.) were prohibited during the intervention period.

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Figure 1: Study design.

We performed two interventions with a cross-over design as follows: (i) ingestion of 25 g of glucose solution (GSI trial) and (ii) ingestion of 25 g of palatinose solution (PSI trial). Each volunteer waited approximately 3 days after completing one trial before crossing over to the next trial. After fasting for 12 h, volunteers visited the laboratory. After resting in the supine position for 10 min, we measured proximal aortic and systemic arterial stiffness, brachial and ankle blood pressure, heart rate (HR), and blood glucose levels at baseline (before ingestion) and 30, 60, and 90 min after ingestion.

Sample size

On the basis of our previous study [5], we performed a power analysis with G*Power 3 to determine the appropriate sample size. As in our previous study, we assumed an effect size for arterial stiffness of 0.5. To detect differences with 80% power and a one-tailed α of 5% using analysis of variance, we calculated that each intervention would require eight individuals. We enrolled 10 participants in the current study.

Body composition

We measured height in units of 0.1 cm using a height gauge and body weight and muscle mass in units of 0.1 kg using a body weight/body composition meter (WB-150 PMA, Tanita, Tokyo, Japan).

Blood pressure and heart rate

Brachial BP and ankle systolic blood pressure (SBP), mean BP (MBP), diastolic BP (DBP), pulse pressure (PP), and HR at rest in the supine position were measured using an automatic oscillometric pulse wave velocity (PWV)/ankle-brachial index device (Omron-Colin, Tokyo, Japan) over the brachial and ankle arteries [15].

Arterial stiffness

In the supine posture, arterial pulse wave velocity (PWV) was measured using a vascular function test device (form PWV/ABI; Colin Medical Technology, Komaki, Japan and VaSera VS-1500AE; Fukuda-Denshi, Tokyo, Japan). In this study, brachial-ankle (ba) PWV and heart-brachial (hb) PWV were measured (Figure 1). For baPWV, we used a blood pressure measurement cuff with a built-in volumetric pressure pulse wave sensor. This cuff was placed around the upper arm and ankle to measure the difference in pulse wave propagation time [15]. For hbPWV, the pulse wave propagation time from the heart to the upper arm was defined as the time difference between the second heart sound and the notch of the brachial artery wave caused by aortic valve closure [16]. baPWV is an index that reflects systemic arterial stiffness and hbPWV reflects aortic arterial stiffness. The cardio-ankle vascular index (CAVI) was automatically calculated from five pulse wave signals using the following formula: CAVI = a [(2ρ/PP) × ln (SBP/DBP) × PWV2] + b, where DBP is diastolic blood pressure, PP is SBP–DBP, ρ is the blood density, and a and b are constants. The CAVI reflects systemic arterial stiffness and is index theoretically adjusted by BP [17].

All measurements were performed while the participants were supine in a quiet room. The daily coefficients of variation were 3 ± 1%, 3 ± 2%, and 3 ± 1% for baPWV, hbPWV, and CAVI, respectively.

Blood glucose

Venous blood was drawn from the subjects’ left fingertip before (baseline) and 30, 60, and 90 min after ingestion of 25 g of glucose or palatinose. BG was measured using the flavin-adenine dinucleotide glucose dehydrogenase method and a Glutest Neo Alpha glucometer (Sanwa Kagaku Kenkyusho, Tokyo, Japan) [18]. The daily coefficient of variation for BG in the laboratory was 3 ± 1%.

Palatinose and glucose ingestion

Each participant orally consumed 25 g of palatinose or 25 g of glucose in 200 ml water within 5 min. The new World Health Organization guideline recommends that adults reduce the daily intake of free sugars to < 25 g [19].

Statistical analysis

All data are shown as mean ± standard deviation (SD). Normality of distribution was assessed with the Shapiro–Wilk test. Data were analyzed using repeated measures two-way analysis of variance (trial × time). Significant differences among means were determined with the Bonferroni post-hoc test. Data were statistically analyzed with IBM SPSS Statistics for Windows v. 25 (IBM, Armonk, NY, USA). P < 0.05 was considered statistically significant.

Results

Figure 2A shows the change (Δ) baPWV before and after ingestion of palatinose and glucose. The ΔbaPWV was signi?cantly increased from baseline at 30, 60, and 90 min (p < 0.01) after ingestion in GSI trial, but not in the PSI trial. The ΔbaPWV was significantly lower at 60 and 90 min (p < 0.05) after ingestion in the PSI trial compared with the GSI trial.

fortune-biomass-feedstock

Figure A:

Figure 2B shows the ΔhbPWV before and after ingestion of palatinose and glucose. The ΔhbPWV was signi?cantly increased from baseline at 30, 60, and 90 min (p < 0.01) after ingestion in GSI trial, but not in the PSI trial. The ΔhbPWV was significantly lower at 30, 60, and 90 min (p < 0.01) after ingestion in the PSI trial compared with the GSI trial.

fortune-biomass-feedstock

Figure B:

Figure 2C shows the ΔCAVI before and after ingestion of palatinose and glucose. The ΔCAVI was signi?cantly increased from baseline at 30, 60, and 90 min (p < 0.01) after ingestion in the GSI trial, but not in the PSI trial. The ΔCAVI was significantly lower at 30, 60, and 90 min (p < 0.05) after ingestion in the PSI trial compared with the GSI trial.

fortune-biomass-feedstock

Figure 2: Changes in arterial stiffness after palatinose or glucose ingestion.

baPWV, brachial­ankle pulse wave velocity; hbPWV, heart­brachial pulse wave velocity; CAVI, cardio-ankle vascular index; GSI, intake of 25 g of glucose solution; ISI, intake of 25 g of palatinose solution.

Values are mean ± SD. **P < 0.01 and *P < 0.05 vs. baseline; ††P < 0.01 and †P < 0.05 vs. the PSI trial.

Table 2 shows baPWV, hbPWV, and the CAVI before and after ingestion of palatinose and glucose. baPWV, hbPWV, and the CAVI were signi?cantly increased from baseline at 30, 60, and 90 min (p < 0.01) after ingestion in the GSI trial, but not in the PSI trial. baPWV and CAVI were not significantly different between trials. hbPWV was significantly lower at 90 min (p < 0.05) after ingestion in the PSI trial compared with the GSI trial.

Baseline

After 30 min

After 60 min

After 90 min

baPWV (cm/sec)

GSI trial

1702.3 ± 149.4

1836.9 ± 171.5**

1841.9 ± 165.3**

1902.5 ± 168.8**

PSI trial

1761.1 ± 165.7

1808.6 ± 181.3

1811.6 ± 177.7

1836.0 ± 178.5

hbPWV (cm/sec)

GSI trial

551.5 ± 53.7

590.9 ± 57.1**

586.4 ± 69.6**

617.4 ± 54.1**

PSI trial

554.6 ± 42.1

562.5 ± 47.3

558.2 ± 42.9

566.5 ± 48.9

CAVI (unit)

GSI trial

8.2 ± 1.0

8.7 ± 1.1**

8.7 ± 1.0**

8.9 ± 1.1**

PSI trial

8.3 ± 1.1

8.4 ± 1.0

8.4 ± 1.2

8.5 ± 1.1

Table 2: Changes in arterial stiffness after palatinose or glucose ingestion Values are mean ± SD.

baPWV, brachial-ankle pulse wave velocity; hbPWV, heart-brachial pulse wave velocity; CAVI, cardio-ankle vascular index; GSI, intake of 25 g of glucose solution; PSI, intake of 25 g of palatinose solution.

**, p<0.01 vs. baseline; †, p<0.05 vs. PSI trial.

Table 3 shows brachial and ankle BP, and HR before and after ingestion of palatinose and glucose. Brachial SBP and MBP were signi?cantly increased from baseline at 90 min (p < 0.05) after ingestion in the GSI trial, but not in the PSI trial. Ankle SBP was signi?cantly increased from baseline at 30, 60, and 90 min (p < 0.01) after ingestion in the GSI trial, but not in the PSI trial. Ankle MBP was signi?cantly increased from baseline at 60 and 90 min (p < 0.01) after ingestion in the GSI trial, but not in the PSI trial. Ankle PP was signi?cantly increased from baseline at 30 (p < 0.01), 60 (p < 0.05), and 90 min (p < 0.05) after ingestion in the GSI trial, but not in the PSI trial. Brachial DBP and PP, ankle DBP, and HR did not change from baseline at 30, 60, and 90 min after ingestion in both trials. Brachial and ankle SBP, MBP, DBP, and PP, and HR did not differ between trials.

Baseline

After 30 min

After 60 min

After 90 min

Brachial SBP (mmHg)

GSI trial

120.5 ± 3.6

125.2 ± 5.8

131.9 ± 5.4

131.6 ± 4.7*

PSI trial

125.6 ± 3.6

127.1 ± 5.0

126.2 ± 4.9

124.0 ± 4.2

Brachial MBP (mmHg)

GSI trial

91.2 ± 3.6

92.6 ± 5.7

97.0 ± 5.2

98.8 ± 5.5*

PSI trial

89.0 ± 3.1

90.8 ± 4.7

95.5 ± 4.4

96.2 ± 4.3

Brachial DBP (mmHg)

GSI trial

73.3 ± 3.2

73.6 ± 4.3

77.3 ± 4.3

78.5 ± 4.6

PSI trial

73.7 ± 3.2

74.3 ± 4.4

76.0 ± 4.5

77.9 ± 4.4

Brachial PP (mmHg)

GSI trial

47.2 ± 2.7

51.6 ± 4.3

54.6 ± 3.5

53.1 ± 3.4

PSI trial

51.9 ± 3.6

52.8 ± 3.5

50.2 ± 4.3

46.1 ± 5.0

Ankle SBP (mmHg)

GSI trial

148.1 ± 7.7

161.0 ± 9.7**

161.4 ± 7.8**

164.4 ± 8.3**

PSI trial

154.4 ± 8.3

156.6 ± 8.8

156.6 ± 8.6

159.6 ± 8.9

Ankle MBP (mmHg)

GSI trial

97.6 ± 4.6

102.4 ± 6.1

104.6 ± 5.3**

107.3 ± 5.0**

PSI trial

101.5 ± 5.4

102.7 ± 5.3

102.5 ± 5.2

104.2 ± 5.9

Ankle DBP (mmHg)

GSI trial

72.4 ± 3.4

73.8 ± 4.4

76.2 ± 4.2

78.7 ± 3.8*

PSI trial

75.0 ± 4.2

75.8 ± 3.7

75.5 ± 4.1

76.5 ± 4.8

Ankle PP (mmHg)

GSI trial

75.7 ± 5.5

87.2 ± 5.3**

85.2 ± 4.6*

85.7 ± 5.8*

PSI trial

79.4 ± 5.3

80.8 ± 5.6

81.1 ± 6.3

83.1 ± 5.4

Heart rate (beats/min)

GSI trial

63.4 ± 2.1

65.1 ± 2.6

63.5 ± 2.1

63.1 ± 2.2

PSI trial

63.1 ± 2.3

62.3 ± 2.4

62.8 ± 2.0

62.7 ± 2.1

Table 3: Blood pressure and heart rate after palatinose or glucose ingestion

Values are mean ± SD.

SBP, systolic blood pressure; MBP, mean blood pressure; DBP, diastolic blood pressure; PP, pulse pressure; GSI, intake of 25 g of glucose solution; PSI, intake of 25 g of palatinose solution.

*, p<0.05 and **, p<0.01 vs. baseline.

Table 4 shows BG levels before and after ingestion of palatinose and glucose. BG levels signi?cantly increased from baseline at 30 (p < 0.01), 60 (p < 0.01), and 90 min (p < 0.05) after ingestion in the GSI trial. BG was signi?cantly increased from baseline at 30 (p < 0.01) and 60 min (p < 0.05) after ingestion in the PSI trial. BG levels were significantly lower at 30 and 60 min (p < 0.01) after ingestion in the PSI trial compared with the GSI trial.

Baseline

After 30 min

After 60 min

After 90 min

Blood glucose (mg/dl)

GSI trial

94.6 ± 4.3

168.0 ± 9.9** ††

163.7 ± 8.8** ††

122.5 ± 12.2*

PSI trial

92.0 ± 2.1

124.1 ± 5.0**

117.4 ± 6.2*

109.2 ± 4.9

Table 4: Blood glucose after palatinose or glucose ingestion

Values are mean ± SD.

GSI, intake of 25 g of glucose solution; PSI, intake of 25 g of isomaltulose solution.

*, p<0.05 and **, p<0.01 vs. baseline. ††, p<0.01 vs. PSI trial.

Discussion

The main novel finding of this study was that baPWV, hbPWV and CAVI were lower after ingestion in the PSI trial than in the GSI trial. These results suggest that transient palatinose intake in older people consumes calories, while suppressing an increase in arterial stiffness.

Postprandial hyperglycemia is a risk factor for cardiovascular disease [20]. Therefore, to prevent cardiovascular disease, the postprandial hyperglycemia needs to be suppressed. A previous study on 10 healthy individuals showed that BG levels after consumption of palatinose led to a slow increase in BG levels after consumption [12]. Our results are consistent with these findings. Therefore, BG levels might not rapidly rise after palatinose ingestion. Consequently, palatinose might be able to moderate the postprandial rise in BG levels compared with other carbohydrates.

Arterial stiffness increases after postprandial hyperglycemia. A previous study, showed that arterial stiffness was elevated after ingestion of sugary foods [21], which is consistent with our findings. We found that the arterial stiffness including the proximal aorta was signi?cantly increased from baseline at 30, 60, and 90 min after ingestion in the GSI trial, but not in the PSI trial. Therefore, palatinose ingestion might be preferable to glucose ingestion, presumably to suppress the increase in arterial stiffness. Diabetes induces peripheral artery disease in all extremities [22], and peripheral arterial stiffness is elevated after glucose ingestion [23]. Previous studies have reported that peripheral arterial stiffness may increase after postprandial hyperglycemia [24]. Therefore, in this situation, it may need to consider systemic arterial stiffness, including peripheral arterial stiffness as well as the aorta. SBP is associated with arterial stiffness [25]. In our study, peripheral SBP and systemic arterial stiffness was signi?cantly increased from baseline at 30, 60, and 90 min after ingestion in the GSI trial, but not in the PSI trial. Therefore, palatinose may be a sugar that suppresses the increase in arterial stiffness associated with elevated SBP after other sugar intakes.

Although the purpose of this study was not to examine the mechanism in which arterial stiffness did not increase after palatinose ingestion involved, we can suggest some hypotheses. We observed an increase in arterial stiffness after glucose ingestion. CAVI is not affected by SBP [26]. Increased CAVI may be due to a decrease in nitric oxide (NO) [27]. Jacome-Sosa et al. [8] stated that “postprandial hyperglycemia is likely to reduce NO bioavailability by limiting arginine availability and increasing asymmetric dimethyl arginine (ADMA) relative to arginine, which is expected to limit substrate availability for NO biosynthesis while competitively inhibiting endothelial NO synthase (eNOS)”. Mah et al. (22) found that after glucose ingestion, vascular endothelial function was decreased, and blood glucose and malondialdehyde levels were increased, but there was no change after ingestion of fructose. Endothelial function is correlated with CAVI [28, 29]. Therefore, the increase in arterial stiffness after glucose ingestion might be related to a decrease in NO production. Future studies to measure NO are required.

In the future, if palatinose is used as a sweetener for cooking, small amount of palatinose may ingest carbohydrates as an energy source and prevent and improve arterial stiffness due to hyperglycemia. Intervention studies are required to determine whether a diet containing small amount of palatinose reduces arterial stiffness.

Limitations of the study

Our study has some limitations. The sample size was small, which limits generalization of our findings. The results of this study cannot be generalized to healthy young people. Additionally, we did not measure changes in glucagon, insulin, growth hormone, and other vasoactive biomarkers, as well as endothelial function, which have important effects on arterial stiffness.

Conclusion

The ΔbaPWV, ΔhbPWV, and ΔCAVI are signi?cantly elevated at 30, 60, and 90 min compared with baseline after ingestion of glucose, but not palatinose. The ΔbaPWV, ΔhbPWV, and ΔCAVI are significantly lower after palatinose intake compared with after glucose intake. Our results suggest that arterial stiffness increases after glucose ingestion, but not after small amount of palatinose intake.

Acknowledgments

We thank all subjects of this study and their team staff. We thank Ellen Knapp, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.

Sources of funding

This work was supported by a research funding from Mitsui Sugar Co., Ltd.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no confict of interest.

Statement of human and animal rights

The present study was in accordance with the declaration of Helsinki and approved by the institutional review board of the Teikyo University of Science (18096), Japan.

Informed consent

Written informed consents were obtained from all participants.

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