Preparation and Evaluation of Polyherbal Extract (Azadirachta indica, Ocimum sanctum, Clitoria ternatea & Hibiscus rosa sinensis) for Antidiabetic Activity

Author(s): Shweta Singh, Dr. Varsha Deva

Diabetes mellitus is a metabolic disorder which has abnormal higher glucose in the blood. The current study was based on the preparation and evaluation of polyherbal extract (Azadirachta indica, Ocimum sanctum, Clitoria ternatea & Hibiscus rosa sinensis) for antidiabetic Activity. The fresh leaves of Azadirachta Indica, Ocimum Sanctum, Hibiscus rosa sinensis L., and fresh flowers of Clitoria ternatea L. were collected from the Prayagraj region, UP East, India. There were authenticated by the botanist at Botanical Survey of India, Allahabad, UP with the reference no. 2023-24/534. The dried leaves of Azadirachta indica, Ocimum sanctum and Hibiscus rosa sinensis L. were rendered into fine powder and extracted using hydroalcoholic (distilled water and ethanol; 1:1) solution through cold maceration process. The leaves of different plants were soaked in a beaker containing hydroalcoholic (distilled water and ethanol; 1:1) solution for 15 days with gradual stirrings, separately. Similarly, dried flowers of Clitoria ternatea L. was rendered into fine powders then soaked into a beaker containing hydroalcoholic (distilled water and ethanol; 1:1) solution for 15 days with gradual stirrings and thus extracted-out. Acute toxicity study was performed. Streptozotocin (STZ) -induced diabetes model was used and evaluated for various parameters i.e., blood glucose level (glucometer), lipid profile, body weights, food consumption, haematological tests, urine analysis, weight of organs and histopathological studies using liver and pancreas. Results showed that Azadirachta indica, Ocimum sanctum, Clitoria ternatea and Hibiscus rosa sinensis have enormous antioxidant and anti-diabetic role as compared with acarbose, ascorbic acid and glibenclamide. It concludes that DM-01, DM-02, DM-03 and DM-04 polyherbal formulations are an effective in the management of diabetes but it’s mode of action is still unknown. So further research may carried-out to confirm its mode of action and optimum doses required in management of DM.