In Vitro Evaluation of The Antioxidant and Anti-Skin Aging Properties of Green Algal Sulfated Polysaccharides
Author(s): Berness Falcao, Jyoti Vishwakarma, Helly Jadav, Sirisha L. Vavilala*
Skin aging is a natural phenomenon witnessed by humans. However various intrinsic and extrinsic factors lead to early skin aging. There have been a variety of approaches to combat skin aging one approach uses antioxidants that are known to fight oxidative stress as well as combat problems of aging. In this study, the antioxidant and anti-skin aging properties of sulfated polysaccharides (SPs) from fresh water microalgae Chlamydomonas reinhardtii (Cr) are evaluated. Sulfated polysaccharides were isolated by hot water extraction method and purified by anion-exchange chromatography. The biochemical composition of the extract showed carbohydrate content of 785.07 mg/g, 324.26 mg/g of sulphate and 393.32 mg/g of uronic acid. These extracts which are enriched with SPs were further used for checking antioxidant and anti-skin aging properties. Cr-SPs showed Superoxide anion scavenging activity of 38-92% at 0.1-2 mg/mL, 51-89% of nitric oxide scavenging ability at 0.2-2 mg/mL, 10-58% of hydrogen peroxide scavenging ability at 1- 10 mg/mL, and 28-68% of ferric ion reducing potential at 0.5-5 mg/mL respectively. Furthermore, Cr-SPs showed 90% anti-elastase enzyme activity at 1 mg/mL, 83% and 89% anti-collagenase and anti-hyaluronidase activities at 1 mg/mL and 2 mg/mL respectively. These promising antioxidant and anti- skin aging properties of Cr-SPs pave way to explore the potential of Cr-SPs in cosmeceutic and pharmaceutical formulations as anti-skin aging agents in a cost-effective manner.