Evaluation of Anti-Bacterial Properties of Euphorbia Condylocarpa Methanol Extract
Author(s): Sepideh Mohammadi, Seyed Ataollah Sadat Shandiz, Razieh Bigdeli, Arash Mahboubi, Mehdi Hedayati, Vahid Asgary
Objective: Infections disease caused by antibiotic resistant bacteria leads to increasingly difficult therapeutic problems. In present study, the antibacterial effect of the Euphorbia Condylocarpa methanol extract against eight pathogenic bacterial strains including: Staphylococcus aureus ATCC 29737, Enterococcus faecalis ATCC 11700, Bacillus subtillis ATCC 6633, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 27853, Staphylococcus epidermidis ATCC 12228, Klebsiella pneumoniae ATCC 10031 were evaluated.
Methods: The total root methanol extract of the Euphorbia condylocarpa was prepared by percolation method. The antibacterial effect was primarily investigated by agar diffusion method followed by determination of the MIC and MBC using broth Macro dilution method. In Addition, the cytotoxicity effect of the extract was evaluated by MTT test using HEK293 cell line.
Results: The highest antibacterial activity of extract against Gram positive bacteria was measured against Staphylococcus epidermidis (22.67 ?0.58mm). The extract had the highest antibacterial effect against Pseudomonas aeuroginosa (MIC 62.50 mg/ml), While, it possess the lowest antibacterial activity against E. coli (MIC 250 mg/ml). The highest MBC values were finding against S. epidermidis, E.coli, E. faecalis, Salmonella typhimurium, and K. pneumonia equal to 500mg/ml. The standard cytotoxicity assays of E. condylocarpa extract showed no toxic effect against HEK293 normal cell line in concentration up to 10 mg/ml.