Comparison of Various Milk Samples Using Spectroscopy Chromatography, and Microscopic Analysis

Author(s): Doneti Ravinder, Pemula Gowtham, Karishma Khatri, Smita C Pawar, Mahendran Botlagunta

Milk is one of the value-added nutritional products for animal health. Different types of milk are being used as a diet for different health benefits. Human milk also being used as trading milk for the nourishment of human health. In diary business, high value-added milk generally mixed with low-value milk for business trading. Consumption of these adulterated dairy products may cause an allergic response in certain individuals. In this study, we analyzed various milk samples, using gas chromatography (GC), Fourier-transform infrared spectroscopy (FT-IR), and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX) to identity the difference among milk samples. FT-IR analysis showed, all milk samples show common wavelength signal at 1384 cm-1 and also distinguish various wavelength spectral patterns (human: 1077 cm-1, donkey: 1092 cm-1, buffalo: 3435 cm-1, cow: 3450 cm-1 and goat: 3443 cm-1. GC analysis results also identified common and distinct profiles for the milk samples tested. SEM-EDAX showed the presence of unique metals such as human milk contains Si-8.82wt%, donkey contains Fe-6.24wt%, and buffalo contains Si-31.29 wt% and surprisingly cow showed a very low percentage of oxygen of about 33.76 wt% when compared to other kinds of milks. Taken together, results confirmed that kinds of milks samples can be distinguished using FT-IR, GC and SEM-EDAX techniques.

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